Preventing Launch at Login
Turning off "Launch at login" in your preferences ( https://docs.tuple.app/article/34-preferences-general) should prevent Tuple from launching on log in. If you find that Tuple is continuing to launch, there may be something else on your machine launching the application.
On mac:
Make sure that under System Preferences -> Users & Groups -> Login Items, Tuple doesn't exist in that list. Make sure that's true for all other users on that computer too (though only root users should make a difference, let's make very sure Tuple isn't getting started some place we don't expect!).
Check to see if Tuple is in the launchctl items. In terminal run:
launchctl list | grep -i tuple
You can then remove the Tuple launch item by running:
launchctl remove app.tuple.app-LaunchAtLoginHelper